Nobiletin improves obesity and insulin resistance in high. Ethanol extract from ulva prolifera prevents high fat diet induced insulin resistance, oxidative stress, and inflammation response in mice. Hyperglycemia, hyperlipidemia, and insulin resistance are hallmarks of obesity induced type 2 diabetes, which is often caused by a high fat diet. Oxidative stress is linked with insulin resistance in the liver 7,10 and the increased level of ros is one of the major causes for insulin resistance 8,9. A high sucrose and high fat hshf diet induces insulin resistance ir and increased. Research article deepure tea improves high fat diet induced insulin resistance and nonalcoholic fatty liver disease jingnadeng, 1,2,3 juanli, 4 hongnamu, 1,2,3,5 yuyingliu, 1,2,3 mingxiawang, 1,2,3 chunshuipan, 1,2,3 jingyufan, 1,2,3 feiye, 4 andjingyanhan 1,2,3,5 tasly microcirculation research center, peking university health science center, beijing, china. High fat diet induced cell proliferation occurs prior to insulin resistance in c57bl6j male mice rockann e. Furthermore, vismodegib, an inhibitor of shh signaling, attenuated erk phosphorylation induced by a highfat diet hfd and restored ppar. Empagliflozin reverses obesity and insulin resistance through. Systemic lowgrade inflammation and intestinal dysfunction are thought to be associated with obesity and ir.
The current article describes the generation and metabolic characterization of high fat diet fed mice as a model of diet induced insulin resistance and obesity. High fat diet induced insulin resistance and elevated retinol binding protein 4 in female rats. Germfree c57bl6j mice are resistant to highfatdiet. Research design and methods after 8 weeks on a high fat diet hfd, c57bl6j mice exhibited obesity, accompanied by insulin resistance and lowgrade chronic inflammation. Research article deepure tea improves high fat dietinduced. In addition, obesity and insulin resistance have been associated with elevated levels of reactive oxygen species ros due to enhanced fatty acid. Our aim in this study was to address whether diet induced insulin resistance plays any roles in the development of aortic and coronary atherosclerosis in hyperlipidemic rabbits. Implications for steatosis and insulin resistance author links open overlay panel eleonora cremonini a b ziwei wang a b ahmed bettaieb c ana m. Estrogens protect against highfat dietinduced insulin.
Doseresponse studies of mitochondrial function using the mtt assay demonstrated relative resistance of huh7 cells to low concentrations diet regimen. Protection of mitochondria prevents highfat dietinduced. Highfathighglucose diet increases fasting glucose and insulin levels in. Research article deepure tea improves high fat diet induced insulin resistance and nonalcoholic fatty liver disease jingnadeng, 1,2,3 juanli, 4 hongnamu, 1,2,3,5 yuyingliu, 1,2,3 mingxiawang, 1,2,3.
Mar 27, 2019 diet plays an essential role in preventing insulin resistance. Insulin resistance, concomitant with substantial weight gain and obesity, can be induced by feeding rodents a high fat diet hfd for a period of many weeks or months 4,5,6,7. Highfat diet induces hepatic insulin resistance and impairment of. Deletion of ppar in adipose tissues of mice protects. Prevention of high fat diet induced insulin resistance in c57bl6j mice by sida rhomboidea roxb. The results showed that gsh and sod levels were decreased, and mda level was increased in glucosamine induced hepg2 cells. Male c57bl6 mice were fed with highfat diet hfd to induce obesity and insulin resistance and treated with 30, 60mgkg silymarin for 18 days.
Fatspecific sirt6 ablation sensitizes mice to highfat. Cytochrome p450 cyp2e1 knockout mice are protected against. Hydroxytyrosol ht may reduce obesity and ir in high fat diet hfdfed mice. Frontiers skeletal muscle mitochondrial bioenergetics and. Diet induced insulin resistance ir adversely affects human health and life span. Fatspecific ablation of sirt6 promotes hfdinduced insulin resistance and hepatic steatosis. Eating foods with a low gi and gl, as well as managing body weight and abdominal fat can reduce the risk. Thus, the current study was conducted to determine if exposure to imidacloprid aggravated high fat diet induced metabolic disorders characterized by adiposity, dyslipidemia, hyperglycemia, and insulin resistance in male c57bl6j mice. Original article fgfr4 prevents hyperlipidemia and.
Drosophila models were used in several recent studies of dietinduced obesity, insulin resistance, hyperglycemia, and hyperinsulinemia 29,30,31,32. Sicyos angulatus prevents highfat dietinduced obesity and. Highfructose and highfat dietinduced insulin resistance enhances atherosclerosis in watanabe heritable hyperlipidemic rabbits bo ning1, xiaoyan wang1,2, ying yu3, ahmed bilal waqar1,qiyu4, tomonari koike3, masashi shiomi3, enqi liu5, yifei wang2 and jianglin fan1,4 abstract. Deletion of ppar in adipose tissues of mice protects against. Glucokinase and irs2 are required for compensatory. Highfat diet hfdinduced obesity is associated with insulin resistance, which may affect brain synaptic plasticity through impairment of. Curcumin prevents high fat diet induced insulin resistance and obesity via attenuating lipogenesis in liver and inflammatory pathway in adipocytes. Insulin resistance ir is a characteristic feature of t2dm, and a significant factor in the development of the disease.
Cytochrome p450 cyp2e1 knockout mice are protected. Pessin1 1depatment of medicine, diabetes research center, albert einstein college of medicine, bronx, new york. Kalupahana 4 obesity research center, university of tennessee, knoxville, tn 37996. Piperine reverses high fat dietinduced hepatic steatosis. A mouse model of dietinduced obesity and insulin resistance. Sicyos angulatus prevents high fat diet induced obesity and insulin resistance in mice. Highfructose and highfat dietinduced insulin resistance. C57bl6 mice were fed with a high fat diet hfd for 8 weeks to induce the metabolic syndrome.
Insulin sensitivity is maintained in both men and mice with high pufa or sfa intake d. Fatspecific sirt6 ablation sensitizes mice to highfat diet. Cohorts of obese mice were continued on the hfd for an additional 8week treatment period with or without empagliflozin. Fatspecific sirt6 ko also increased inflammation in the adipose tissue, which may have contributed to insulin resistance in highfat dietfed fko mice. Conclusions iapp induced er stress distinguishes the successful versus unsuccessful islet adaptation to a high fat diet in rats. Abstract recent studies showed that germfree gf mice are resistant to obesity when consuming a highfat, highcarbohydrate western diet. B pathway, insulin resistance, palmitate, highfat diet background obesity is regarded as the major contributing factor in the development of insulin resistance li et al.
Pdf edible birds nest prevents high fat dietinduced. High fathighglucose diet increases fasting glucose and insulin levels in. Consumption of high fat diet and sugary drink hfs leads to abnormal metabolic phenotype in mice. Increased oxidative stress precedes the onset of highfat. Obese mice were fed a hfd for 8 weeks and then treated without hfd control group or with nob at 10 or 100 mgkg. Hydroxytyrosol improves obesity and insulin resistance by modulating gut microbiota in highfat dietinduced obese mice zhuoqun liu, 1 ningning wang, 1 yanan ma. The faseb journal research communication germfree c57bl6j mice are resistant to highfatdietinduced insulin resistance and have altered cholesterol metabolism sylvie rabot,1 mathieu membrez,1 aure. However, because the lowgrade inflammatory tone induced by high fat feeding initiates insulin resistance and glucose intolerance 2, 3 and because inflammatory cytokines are known to impair insulin signaling, the proinflammatory effect of estrogens should be considered as controversial.
Original article chinese medicine jinlida jld ameliorates. Dietary chickpeas reverse visceral adiposity, dyslipidaemia and insulin resistance in rats induced by a chronic high fat diet volume 98 issue 4 ying yang, libin zhou, yuanjun gu, yibo zhang, jingfeng tang, fengying li, wenbin shang, boren jiang, xiaohua yue, mingdao chen. Ss31 had no effect on weight gain, insulin resistance or hyperglycemia. Nobiletin improves obesity and insulin resistance in highfat. Mckeehan objectivefibroblast growth factor fgf family signaling largely controls cellular homeostasis through shortrange intercell paracrine communication. Ko would increase insulin signaling and brown fat functionality, conferring resistance to diet induced obesity and its associated metabolic complications. Hfdinduced insulin resistance through inhibition of the nf. Combination of nutritional polyphenols supplementation. Successful versus failed adaptation to highfat diet.
In genetically susceptible animals, research has shown that simply offering them a diet high in fat and refined carbohydrate leads to overeating and the rapid induction of insulin resistance ir within just a few days. Hydroxytyrosol improves obesity and insulin resistance by. The suppression of atgl in fko mice was accounted for by the increased phosphorylation and acetylation of foxo1, which compromises the transcriptional activity of this positive regulator of atgl. Characterization of inflammation and insulin resistance in high. Gbb signaling provokes insulin resistance through the tribbles expression seunghyun hong, 1 moonyoung kang, 1, 2 kyusun lee, a, 1, 2 and kweon yu b, 1, 2, 3 1 neurophysiology and metabolism research group, korea research institute of bioscience and biotechnology kribb, daejeon 34141, korea. Increasing evidence links intestinal bacteria to development of diet induced obesity dio. Methods six weeks old mice were initiated on regular diet rd or hfd providing 60 kcal energy from fat for 9. High fathigh glucose diet induces metabolic syndrome in an. Aug 12, 2015 individuals with insulin resistance and resulting impaired glucose tolerance along with type 2 diabetes showed an increased prevalence of atherosclerosis. Diet to prevent and reverse insulin resistance and type 2. We report on effects of the vegetable on a hf diet induced fat accumulation, insulin resistance, inflammation, endotoxemia and the expression of genes encoding proteins that are involved or. Edible birds nest prevents high fat diet induced insulin resistance in rats.
The mouse model of diet induced obesity has become one of the most important tools for understanding the interplay of high fat western diets and the development of obesity. The diet induced obesity model closely mimics the increasingly availability of the high fat high density foods in modern society over the past two decades, which are main. Dietary chickpeas reverse visceral adiposity, dyslipidaemia. However, it remains unclear what mechanisms are involved in the antiobesity phenotype and whether gf mice develop insulin resistance and. In this study, we examined the effects of nob on obesity and insulin resistance, and the underlying mechanisms, in high fat diet hfd induced obese mice. Sonic hedgehog signaling instigates highfat dietinduced. Eicosapentaenoic acid prevents and reverses insulin resistance in high fat diet induced obese mice via modulation of adipose tissue inflammation. Probiotics improve high fat dietinduced hepatic steatosis. Imidacloprid, a neonicotinoid insecticide widely used in agriculture worldwide, has been reported to promote adipogenesis and cause insulin resistance in vitro. Two opposite views are debated on mechanisms by which mitochondrial function could be involved in skeletal muscle ir. Dietinduced insulin resistance ir adversely affects human health and life span. Pdf curcumin prevents high fat diet induced insulin. We fed watanabe heritable hyperlipidemic whhl rabbits with a. However, ss31 prevented loss of glomerular endothelial.
Dec 11, 2017 additionally, prolonged administration of high fat diet fed mice with the inos inhibitor aminoguanidine fully restored the obesity induced insulin resistance and significantly attenuated the. Highfat diet, muscular lipotoxicity and insulin resistance. Eicosapentaenoic acid prevents and reverses insulin. Eicosapentaenoic acid prevents and reverses insulin resistance in high fat diet induced obese mice via modulation of adipose tissue inflammation nishan s. Resistance to highfat dietinduced obesity and insulin. High fat diet feeding induced insulin resistance exacerbates. Epicatechin protects the intestinal barrier from high fat. Ceramidemediated hepatotoxicity and insulin resistance. We show that musclespecific overexpression of human mitochondrial transcription factor a tfam attenuates highfat diet hfdinduced fat gain and ir in mice in conjunction with increased energy expenditure and reduced oxidative stress. For development of insulin resistance, we fed the male wistar rats with high fat diet hfd for eight weeks, followed by 6hydroxydopamine 6ohda administration, a toxin widely used for pd induction, in medial forebrain bundle mfb of rats. Gtt a and itt b in loxp and fko mice with hfd n 15. Curcumin prevents high fat diet induced insulin resistance and obesity via attenuating lipogenesis in liver and inflammatory pathway in adipocytes article pdf available in plos one 71.
After 12 weeks of hfd, the mice that exhibited insulin resistance then received 5 weeks of apocynin 2. In drosophila larvae, a highsugar diet induces type 2 diabetic phenotypes including hyperglycemia, high tg, and insulin resistance 31. Deepure tea improves high fat dietinduced insulin resistance. Mechanisms preserving insulin action during high dietary fat intake. The affect of jld on blood glucose, lipid, insulin, adiponectin, alanine aminotransferase alt, aspartate aminotransferase ast and. Apocynin improves insulin resistance through suppressing. Mar 04, 2019 insulin resistance ir is one of the most frequent complications of obesity. We show that musclespecific overexpression of human mitochondrial transcription factor a tfam attenuates high fat diet hfd induced fat gain and ir in mice in conjunction with increased energy expenditure and reduced oxidative stress. Kitajima et al overexpression of lipoprotein lipase improves insulin resistance induced by a high fat diet 1203. Characterization of in vivo glucose dynamics pointed to improved hepatic glucose metabolism as the basis for preventing high fat diet induced insulin resistance. Fgfr4 prevents hyperlipidemia and insulin resistance but underlies high fat diet induced fatty liver xinqiang huang, chaofeng yang, yongde luo, chengliu jin, fen wang, and wallace l. Highfat feeding caused insulin resistance in skeletal muscle that was associated with significantly. It further features detailed protocols to perform the oral glucose tolerance test and the insulin tolerance test, monitoring wholebody alterations of glucose metabolism in vivo. Jan 20, 2016 it has been suggested that skeletal muscle mitochondria play a key role in high fat hf diet induced insulin resistance ir.
Overexpression of lipoprotein lipase improves insulin. High fructose and high fat diet induced insulin resistance enhances atherosclerosis in watanabe heritable hyperlipidemic rabbits bo ning1, xiaoyan wang1,2, ying yu3, ahmed bilal waqar1,qiyu4, tomonari koike3, masashi shiomi3, enqi liu5, yifei wang2 and jianglin fan1,4 abstract. Cytochrome p450 cyp2e1 knockout mice are protected against high fat diet induced obesity and insulin resistance haihong zong,1 michal armoni,2 chava harel,2 eddy karnieli,2 and jeffrey e. To study the physiological role of grasp55, the grasp55 gene in mouse was inactivated by replacing. The high fructose and high fat diet hffd group n 14 was fed a chow diet supplemented with 30 % fructose and 10 % coconut oil 91 % saturated fatty acids by weight because hffd diet can induce more prominent insulin resistance than a diet containing fructose alone. Imidacloprid promotes high fat diet induced adiposity and insulin resistance in male c57bl6j mice article pdf available in journal of agricultural and food chemistry 6449 november 2016. Characterization of inflammation and insulin resistance in. Insulin resistance, concomitant with substantial weight gain and obesity, can be induced by feeding rodents a high fat diet hfd for a period of. The mitochondrial protein uncoupling protein 3, which is induced under high fat conditions, may serve to protect mitochondria against lipid induced oxidative damage, but is reduced in the prediabetic state. Most prior studies have focused on adipose tissue as the source of obesityassociated inflammation. Hfdfed mice were injected with glucose 2 gkg or insulin 1 ukg, and blood glucose was measured at indicated time points by a glucose meter. Combination of nutritional polyphenols supplementation with exercise training counteracts insulin resistance and improves endurance in high fat diet induced. The 6ohda treatment resulted in neuronal damage and loss of striatal dopamine level.
However, bone marrow graft experiments indicated that. Jci glucokinase and irs2 are required for compensatory. A randomized controlled experiment was conducted to provide evidence in support of the affects of jld on insulin resistance induced by hfd. High fat dietinduced obesity is associated with insulin resistance ir and other chronic. We investigated the effects of apocynin on high fat diet hfd induced insulin resistance in c57bl6 mice. Additionally, prolonged administration of high fat diet fed mice with the inos inhibitor aminoguanidine fully restored the obesity induced insulin resistance and significantly attenuated the. Ethanol extract from ulva prolifera prevents highfat diet. Request pdf increased oxidative stress precedes the onset of highfat dietinduced insulin resistance and obesity insulin resistance is a key pathophysiological feature of metabolic syndrome. Highfat dietinduced obesity impairs insulin signaling in. Unraveling the temporal pattern of dietinduced insulin resistance.
The purpose of the current study was to determine the effects of imidacloprid and its interaction with dietary fat in the development of adiposity and insulin resistance using male c57bl6j mice. Oral probiotic treatment significantly improved the high fat diet induced hepatic nkt cell depletion, insulin resistance and hepatic steatosis. Individuals with insulin resistance and resulting impaired glucose tolerance along with type 2 diabetes showed an increased prevalence of. Highfructose and highfat dietinduced insulin resistance enhances. Characterization of in vivo glucose dynamics pointed to improved hepatic glucose metabolism as the basis for preventing highfat dietinduced insulin resistance. Carboneau,4 kavin arasi,2 kirk pappan,7 vincent poitout,5,6 and maureen gannon1,2,3,4 1department of veterans affairs tennessee valley, nashville, tennessee. Over the experimental period, mechanical sensitivity was evaluated at the. Insulin resistance ir is one of the most frequent complications of obesity.409 420 873 503 176 1461 769 774 1638 879 1141 364 1116 844 1488 43 1108 1088 992 1076 82 482 121 210 443 619 271 837 1393 279